03/07/2020
The Human Resources Strategy for Researchers

PhD stipend

This job offer has expired


  • ORGANISATION/COMPANY
    Institute of Fundamental Technological Research Polish Academy of Sciences
  • RESEARCH FIELD
    Biological sciences
    Computer science
    Mathematics
    Physics
  • RESEARCHER PROFILE
    First Stage Researcher (R1)
  • APPLICATION DEADLINE
    15/08/2020 12:00 - Europe/Brussels
  • LOCATION
    Poland › Warsaw
  • TYPE OF CONTRACT
    Temporary
  • JOB STATUS
    Other
  • OFFER STARTING DATE
    01/09/2020

OFFER DESCRIPTION

The position is offered within the GRIEG project funded from the Norway and EEA Grants 2014‑2021 under the Basic Research Programme operated by the National Science Centre (Poland).

 

Title: Interaction of natural killer cells with an infected cancer cell population: microfluidics-based experiments and single-cell-level mathematical modeling.

 

Principal Investigator: prof. Tomasz Lipniacki http://pmbm.ippt.pan.pl

Project duration: 36 months

Institution: Institute of Fundamental Technological Research, Polish Academy of Sciences

The project will be conducted in cooperation with the Norwegian Institute SINTEF in Trondheim.

 

PROJECT OBJECTIVES: The aim of the project is to analyze interactions of natural killer (NK) cells with a virus infected cell population at the single-cell resolution. Specifically, we will investigate the interactions of NK cells, two respiratory epithelial cell lines: cancerous A549 and non-cancerous BEAS-2B, and two respiratory viruses: respiratory syncytial virus (RSV) and influenza A virus (IAV).

 

The main objectives are as follows:

  • Elucidation of the impact of NK cell-induced cell death on the dynamics of propagation and eradication of RSV and IAV infections. Characterization of distinct levels of NK cells’ activity towards the infected and the noninfected subpopulation of the cell culture.
  • Determination of the capacity of NK cells to induce immunogenic cell death in infected cells.
  • Development of a droplet-based microfluidic system to perform experiments on co-cultures of adherent and suspended cells, that will enable data acquisition at the single-cell resolution.
  • Development of an agent-based, spatial stochastic model capturing interactions of NK cells with an infected cell population.

 

TECHNIQUES: We will integrate the following techniques:

  • Live confocal imaging using viruses encoding fluorescent proteins, cells expressing fluorescently tagged transcription factors and fluorescent markers of apoptosis.
  • Immunostaining scanning. In this technique we simultaneously observe different triplets or quadruplets of proteins of a considered regulatory pathway in single cells. We will also use RNA FISH and the emerging technique of sequential immunostaining.
  • Gene expression, protein analysis and cytokine production in infected cell populations through the use of RT-PCR, digital PCR, Western Blot. ELISA and other relevant techniques
  • Droplet-based microfluidic systems to culture adherent and suspended cells allowing for their confocal imaging and immunostaining.

Mathematical modeling employing Markov processes to describe virus replication, intra- and intercellular signaling, NK cells activation, eradication of infected (and non-infected) cells in heterogeneous cell populations. We will account for spatial aspects of the cytokine diffusion and viral spread.

More Information

Benefits

We offer 5000PLN/month stipend. Some additional support can be obtained from the doctoral school as TIB PAN http://ibib.waw.pl/en/doctoral-schools/tib-pan

Selection process

Additional comments

Required documents

  1. Copy of MSc diploma. Bachelor diplomas may be also included for evaluation.
  2. CV containing information about:
  • Publications,
  • Conference presentations,
  • Prizes and stipends,
  • As well as other information that can help to evaluate, experience, achievements, and scientific standing of the candidate.

 

Please include in your CV the following clause: "I agree to the processing of personal data contained in my job offer for the needs necessary to carry out the recruitment process conducted by IPPT PAN with headquarters in Warsaw, ul. A. Pawińskiego 5B, according to art. 13 para. 1 and 2 of Regulation (EU) 2016/679 of the Parliament and of the Council of 27 April 2016 on the protection of individuals with regard to the processing of personal data and the free movement of such data and the repeal of Directive 95/46 / EC (RODO).

 

The documents should be sent till August 15, 2020 to the Project PI, prof. Tomasz Lipniacki tlipnia@ippt.pan.pl with a copy to Department Secretary Ms. Agnieszka Ponarska aponar@ippt.pan.pl. If needed the candidates will be contacted and invited for the interview. The final decisions will be taken before Oct 1, 2020

Required Research Experiences

  • RESEARCH FIELD
    Biological sciences
  • YEARS OF RESEARCH EXPERIENCE
    1 - 4
  • RESEARCH FIELD
    Mathematics
  • YEARS OF RESEARCH EXPERIENCE
    1 - 4
  • RESEARCH FIELD
    Computer science
  • YEARS OF RESEARCH EXPERIENCE
    1 - 4
  • RESEARCH FIELD
    Physics
  • YEARS OF RESEARCH EXPERIENCE
    1 - 4

Offer Requirements

  • REQUIRED EDUCATION LEVEL
    Biological sciences: Master Degree or equivalent
    Physics: Master Degree or equivalent
    Computer science: Master Degree or equivalent
    Mathematics: Master Degree or equivalent
  • REQUIRED LANGUAGES
    ENGLISH: Excellent

Skills/Qualifications

We seek candidates having basic experience in one or two of the following areas:

  1. Immunology, virology or cancer cell biology, with basic knowledge in experimental techniques: cell culture, RT or digital PCR, Western blot, live and fixed-cell confocal imaging, cell sorting. Experience in other methods planned for the project and described above is desirable.
  2. Mathematical and numerical system biology, ordinary and partial differential equations, stochastic processes, programming.
  3. Microfluidic fabrication and droplet microfluidic techniques.

 

Background in two of above areas will be considered an additional benefit.

Specific Requirements

According to National Science Centre regulations candidate must became a “participant in a doctoral program” or a “doctoral candidate at a doctoral school”, such as TIB PAN http://ibib.waw.pl/en/doctoral-schools/tib-pan

 

Candidate should meet criteria given in:

https://www.ncn.gov.pl/sites/default/files/pliki/regulaminy/grieg_guide_for_applicants.pdf

Map Information

Job Work Location Personal Assistance locations
Work location(s)
2 position(s) available at
Institute of Fundamental Technological Research Polish Academy of Sciences
Poland
Mazovia
Warsaw
02-106
A.Pawińskiego 5B

EURAXESS offer ID: 538868

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